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chromatin immunoprecipitation (chip)-it ® express magnetic chip kit & sonication shearing kit  (Active Motif)


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    Active Motif chromatin immunoprecipitation (chip)-it ® express magnetic chip kit & sonication shearing kit
    Chromatin Immunoprecipitation (Chip) It ® Express Magnetic Chip Kit & Sonication Shearing Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip-it+express+sonication+kit/chip+it+express+chromatin+immunoprecipitation+kit/pmc07862576-108-1-19
    Average 90 stars, based on 1 article reviews
    chromatin immunoprecipitation (chip)-it ® express magnetic chip kit & sonication shearing kit - by Bioz Stars, 2026-09
    90/100 stars

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    Chromatin Immunoprecipitation:

    Article Title: Progesterone Receptor Attenuates STAT1-Mediated Interferon Signaling in Breast Cancer
    Article Snippet: Relative concentrations were quantified using the LightCycler96 (Roche, Software 1.1, Absolute Quantification Analysis), using a 6-point standard curve. .. ChIP was performed using the ChIP-IT Express Kit (Active Motif) according to manufacturer’s instructions using sonication for chromatin shearing. .. Lysates were immunoprecipitated (IP) overnight (18hr) with the following antibodies: STAT1 (Santa Cruz, sc-346), STAT2 (Santa Cruz, sc-476), IRF9 (Santa Cruz, sc-10793), or an equal amount of negative control mouse or rabbit IgG.

    Article Title: Search for novel STAT3-dependent genes reveals SERPINA3 as a new STAT3 target that regulates invasion of human melanoma cells.
    Article Snippet: .. Overall, 1 × 107 cells were fixed with 1% formaldehyde for 20 min, and sonicated for number of pulses and time using Diagenode sonicator with components of ChIP IT Express kit (Active Motif, USA). .. DNA fragmentation was evaluated using Agilent Bioanalyzer.

    Article Title: Interaction between MUC1 and STAT1 drives IFITM1 overexpression in aromatase inhibitor-resistant breast cancer cells and mediates estrogen-induced apoptosis
    Article Snippet: Immune complexes were washed three times with PBS, resuspended in Laemmli sample buffer containing dithiothreitol and β-mercaptoethanol (Invitrogen, Cat#NP0007), boiled for 5 min, and subjected to western blotting analysis. .. ChIP was performed using the ChIP-IT Express Kit (Active Motif, Cat53008) according to the manufacturer’s instructions using sonication as the method for chromatin shearing. .. Lysates were immunoprecipitated (IP) overnight (18 h) with the following antibodies anti-STAT1 (Santa Cruz Biotechnology, Cat#SC-464), anti-STAT2 (Santa Cruz Biotechnology, Cat#SC-514193), anti-MUC1 (Santa Cruz Biotechnology, Cat#SC-7313) or an equal amount of mouse (Santa Cruz Biotechnology, Cat#SC-2762) or rabbit IgG (Santa Cruz Biotechnology, Cat#SC-2027).

    Article Title: Enhanced IFNα Signaling Promotes Ligand-Independent Activation of ERα to Promote Aromatase Inhibitor Resistance in Breast Cancer
    Article Snippet: .. ChIP was performed after sonication using the ChIP-IT Express Kit (Active Motif, Carlsbad, CA, USA, Cat53008) according to the manufacturer’s instructions. .. Lysates were immunoprecipitated (IP) overnight (18 h) with the following antibodies: anti-STAT1 (Cell Signaling, Cat#9172S), anti-ERα (Cell Signaling, Cat#8644S) or an equal amount of rabbit IgG (Santa Cruz Biotechnology, Cat#SC-2027).

    Article Title: Progesterone Receptor Attenuates STAT1-Mediated Interferon Signaling in Breast Cancer
    Article Snippet: Relative concentrations were quantified using the LightCycler96 (Roche, Software 1.1, Absolute Quantification Analysis), using a 6-point standard curve. .. ChIP assays ChIP was performed using the ChIP-IT Express Kit (Active Motif) according to manufacturer’s instructions using sonication for chromatin shearing. .. Lysates were immunoprecipitated (IP) overnight (18hr) with the following antibodies: STAT1 (Santa Cruz, sc-346), STAT2 (Santa Cruz, sc-476), IRF9 (Santa Cruz, sc-10793), or an equal amount of negative control mouse or rabbit IgG.

    Sonication:

    Article Title: Progesterone Receptor Attenuates STAT1-Mediated Interferon Signaling in Breast Cancer
    Article Snippet: Relative concentrations were quantified using the LightCycler96 (Roche, Software 1.1, Absolute Quantification Analysis), using a 6-point standard curve. .. ChIP was performed using the ChIP-IT Express Kit (Active Motif) according to manufacturer’s instructions using sonication for chromatin shearing. .. Lysates were immunoprecipitated (IP) overnight (18hr) with the following antibodies: STAT1 (Santa Cruz, sc-346), STAT2 (Santa Cruz, sc-476), IRF9 (Santa Cruz, sc-10793), or an equal amount of negative control mouse or rabbit IgG.

    Article Title: Search for novel STAT3-dependent genes reveals SERPINA3 as a new STAT3 target that regulates invasion of human melanoma cells.
    Article Snippet: .. Overall, 1 × 107 cells were fixed with 1% formaldehyde for 20 min, and sonicated for number of pulses and time using Diagenode sonicator with components of ChIP IT Express kit (Active Motif, USA). .. DNA fragmentation was evaluated using Agilent Bioanalyzer.

    Article Title: Interaction between MUC1 and STAT1 drives IFITM1 overexpression in aromatase inhibitor-resistant breast cancer cells and mediates estrogen-induced apoptosis
    Article Snippet: Immune complexes were washed three times with PBS, resuspended in Laemmli sample buffer containing dithiothreitol and β-mercaptoethanol (Invitrogen, Cat#NP0007), boiled for 5 min, and subjected to western blotting analysis. .. ChIP was performed using the ChIP-IT Express Kit (Active Motif, Cat53008) according to the manufacturer’s instructions using sonication as the method for chromatin shearing. .. Lysates were immunoprecipitated (IP) overnight (18 h) with the following antibodies anti-STAT1 (Santa Cruz Biotechnology, Cat#SC-464), anti-STAT2 (Santa Cruz Biotechnology, Cat#SC-514193), anti-MUC1 (Santa Cruz Biotechnology, Cat#SC-7313) or an equal amount of mouse (Santa Cruz Biotechnology, Cat#SC-2762) or rabbit IgG (Santa Cruz Biotechnology, Cat#SC-2027).

    Article Title: Enhanced IFNα Signaling Promotes Ligand-Independent Activation of ERα to Promote Aromatase Inhibitor Resistance in Breast Cancer
    Article Snippet: .. ChIP was performed after sonication using the ChIP-IT Express Kit (Active Motif, Carlsbad, CA, USA, Cat53008) according to the manufacturer’s instructions. .. Lysates were immunoprecipitated (IP) overnight (18 h) with the following antibodies: anti-STAT1 (Cell Signaling, Cat#9172S), anti-ERα (Cell Signaling, Cat#8644S) or an equal amount of rabbit IgG (Santa Cruz Biotechnology, Cat#SC-2027).

    Article Title: Progesterone Receptor Attenuates STAT1-Mediated Interferon Signaling in Breast Cancer
    Article Snippet: Relative concentrations were quantified using the LightCycler96 (Roche, Software 1.1, Absolute Quantification Analysis), using a 6-point standard curve. .. ChIP assays ChIP was performed using the ChIP-IT Express Kit (Active Motif) according to manufacturer’s instructions using sonication for chromatin shearing. .. Lysates were immunoprecipitated (IP) overnight (18hr) with the following antibodies: STAT1 (Santa Cruz, sc-346), STAT2 (Santa Cruz, sc-476), IRF9 (Santa Cruz, sc-10793), or an equal amount of negative control mouse or rabbit IgG.

    other:

    Article Title: Mdfi Promotes C2C12 Cell Differentiation and Positively Modulates Fast-to-Slow-Twitch Muscle Fiber Transformation
    Article Snippet: The chromatin immunoprecipitation (ChIP)-IT ® Express Magnetic ChIP kit & sonication shearing kit (Catalog number 53008) was purchased from Active Motif (Carlsbad, CA, United States).

    Article Title: Loss of H3K9 trimethylation alters chromosome compaction and transcription factor retention during mitosis.
    Article Snippet: Then, 50 μl of this sheared chromatin was used to assess DNA concentration and sonication efficiency (typically DNA between 200 and 800 bp) using ChIP-IT Express kit (Active motif) and ChIP clean & concentrator kit (Zymo research).

    Article Title: Transcriptional regulation of ETV5 by mitogen-activated protein kinase via ETS-1 in human pancreatic cancer cells.
    Article Snippet: The collected cells were sonicated in a Biorupter (Cosmobio, Tokyo, Japan) and immunoprecipitated with monoclonal anti-ETS-1 antibody (clone D8O8A; Cell signaling Technology) or Normal Rabbit IgG, a nonspecific immunoglobulin (Cell signaling Technology) using ChIP-IT Express kit (Active Motif, Carlsbad, CA, USA).



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